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High-performance Liquid Chromatographic Assay of L-alpha-glycerophosphorylcholine Using a Two-Step Enzymic Conversion

G Abbiati, T Fossati, M Arrigoni, P Rolle, G L Dognini, C Castiglioni

J Chromatogr. 1991 May 31;566(2):445-51.

PMID: 1658023

Abstract:

A high-performance liquid chromatographic method using an enzymic reactor for determination of L-alpha-glycerophosphorylcholine in pharmaceutical forms is described. The procedure includes incubation of L-alpha-glycerophosphorylcholine with glycerophosphorylcholine phosphodiesterase (EC 3.1.4.2), giving choline and glycerophosphate, and subsequent chromatography of choline with a post-column enzymic reactor and electrochemical detection. The results obtained show a close linearity of the whole assay from 2 to 150 nmol/ml L-alpha-glycerophosphorylcholine, the sensitivity being 2 pmol per 20 microliters of injected sample. The precision of the method in the analysis of L-alpha-glycerophosphorylcholine in pharmaceutical forms, ampoules and capsules, was 1.34 and 1.21%, respectively.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42412154 L-α-Glycerophosphorylcholine L-α-Glycerophosphorylcholine Price
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