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High Performance Liquid Chromatographic Determination of Folic Acid and Its Photodegradation Products in the Presence of Riboflavin

High Performance Liquid Chromatographic Determination of Folic Acid and Its Photodegradation Products in the Presence of Riboflavin

M J Akhtar, M A Khan, I Ahmad

J Pharm Biomed Anal. 1997 Sep;16(1):95-9.

PMID: 9447556

Abstract:

A high performance liquid chromatographic procedure was developed to determine folic acid and its photodegradation products, p-aminobenzoic acid, pterine-6-carboxylic acid, p-aminobenzoyl-L-glutamic acid, and pteroic acid in the presence of riboflavin. The method involves reversed phase, paired-ion chromatography on mu-BondaPak C18 column using a UV detector (254 nm), and isocratic solvent system (at ambient temperature) comprising 0.017 M monobasic potassium phosphate, tetrabutyl ammonium hydroxide solution (20%, aqueous) and methanol (870:15:250, v/v). The range of quantitation for the individual compounds was found to be: p-aminobenzoic acid, 0.01 - 1.25 x 10(-5) M; pterine-6-carboxylic acid, 0.01-2.0 x 10(-5) M; p-aminobenzoyl-L-glutamic acid, 0.02-2.0 x 10(-5) M; pteroic acid, 0.02-2.5 x 10(-5) M; folic acid, 1.0-5.0 x 10(-5) M; riboflavin, 1.0-5.0 x 10(-5) M. Linear regression analysis of the data demonstrates adequate performance of the method in terms of accuracy and precision (R. S. D. 3%). The method is specific, rapid and convenient and has been applied to photodegradation studies of folic acid in the presence and absence of riboflavin.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP948607	Pterine-6-carboxylic acid		948-60-7	Price

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