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High-performance Liquid Chromatographic Method for the Determination and Pharmacokinetic Study of Dehydrotumulosic Acid in the Plasma of Rats Having Taken the Traditional Chinese Medicinal Preparation Ling-Gui-Zhu-Gan Decoction

High-performance Liquid Chromatographic Method for the Determination and Pharmacokinetic Study of Dehydrotumulosic Acid in the Plasma of Rats Having Taken the Traditional Chinese Medicinal Preparation Ling-Gui-Zhu-Gan Decoction

Zonghua Song, Kaishun Bi, Xu Luo

J Chromatogr B Analyt Technol Biomed Life Sci. 2003 May 25;788(2):387-91.

PMID: 12705979

Abstract:

A high-performance liquid chromatographic method for the determination of dehydrotumulosic acid in plasma of rats having been administrated orally with the traditional Chinese medicinal preparation Ling-Gui-Zhu-Gan decoction was developed. Plasma samples taken from rats were acidified with hydrochloric acid and extracted with ethyl acetate. Separation of the main effective constituent dehydrotumulosic acid was accomplished on a C(18) stationary phase and a mobile phase of methanol-acetonitrile-2% glacial acetic acid (13:12:10, v/v), with a UV detector setting at 242 nm. After validation, the method was used for preliminary investigation of the pharmacokinetic profiles of dehydrotumulosic acid administrated in Ling-Gui-Zhu-Gan decoction.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP6754161	Dehydrotumulosic acid		6754-16-1	Price

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