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High-performance Liquid Chromatographic Method for the Determination and Pharmacokinetic Study of Oxypeucedanin Hydrate and Byak-Angelicin After Oral Administration of Angelica Dahurica Extracts in Mongrel Dog Plasma

High-performance Liquid Chromatographic Method for the Determination and Pharmacokinetic Study of Oxypeucedanin Hydrate and Byak-Angelicin After Oral Administration of Angelica Dahurica Extracts in Mongrel Dog Plasma

Ying Xie, Yi Chen, Mei Lin, Jun Wen, Guorong Fan, Yutian Wu

J Pharm Biomed Anal. 2007 May 9;44(1):166-72.

PMID: 17344014

Abstract:

A high-performance liquid chromatographic method was developed and validated for the determination and pharmacokinetic study of oxypeucedanin hydrate and byak-angelicin after oral administration of Angelica dahurica extracts in mongrel dog plasma. The coumarin components and the internal standard isopsoralen were extracted from plasma samples with the mixture of tert-butyl methyl ether and n-hexane (4:1, v/v). Chromatographic separation was performed on a C(18) column (200 mm x 4.6mm, 5 microm) with the mobile phase acetonitrile-methanol-water-acetic acid (20:15:65:2, v/v/v/v) at a flow-rate of 1.0 ml/min. Only the peak of oxypeucedanin hydrate and byak-angelicin could be detected in dog plasma after oral administration of ethanol extracts of A. dahurica mainly containing xanthotoxol, osthenol, imperatorin, oxypeucedanin hydrate and byak-angelicin. The calibration curves of oxypeucedanin hydrate and byak-angelicin were linear over a range of 22.08-8830.00 and 6.08-2430.00 ng/ml in dog plasma, respectively. The quantification limit of oxypeucedanin hydrate and byak-angelicin in dog plasma was 22.08 and 6.08 ng/ml, respectively. The intra- and inter-day precision was less than 7.6% and 8.5% and the accuracy was from 91.9% to 106.1%. The lowest absolute recoveries of oxypeucedanin hydrate and byak-angelicin were 85.7% and 87.0%, respectively. The method was successfully applied to the pharmacokinetic studies of oxypeucedanin hydrate and byak-angelicin in dog plasma after oral administration of ethanol extracts from A. dahurica.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP737520	Oxypeucedanin		737-52-0	Price

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