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High-performance Liquid Chromatographic Quantitation of Trimethoprim, Sulfamethoxazole, and N4-acetylsulfamethoxazole in Body Fluids

A Weber, K E Opheim, G R Siber, J F Ericson, A L Smith

J Chromatogr. 1983 Dec 9;278(2):337-45.

PMID: 6668314

Abstract:

We describe a rapid, precise and simple procedure for the quantitative determination of trimethoprim, sulfamethoxazole, and N4-acetylsulfamethoxazole in body fluids by reversed-phase high-performance liquid chromatography. This method utilizes antipyrine as an internal standard with the compounds detected by dual-wavelength monitoring at 225 nm and 254 nm after a single-step extraction. Precision, sensitivity, and accuracy of this assay are within the range of clinical utility; the coefficient of variation is less than or equal to 3%, sensitivity less than 0.5 micrograms/ml for all compounds, and recovery greater than 97%. The short time for performance and small sample size makes the assay ideal for clinical drug monitoring and pharmacokinetic studies.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP21312107-A N4-Acetylsulfamethoxazole N4-Acetylsulfamethoxazole 21312-10-7 Price
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