0

High-performance Liquid Chromatography Assays for Desmethoxyyangonin, Methysticin, Kavain and Their Microsomal Metabolites

Shuang Fu, Bruce N Tattam, Colin C Duke, Iqbal Ramzan

Biomed Chromatogr. 2009 Jan;23(1):81-91.

PMID: 18661482

Abstract:

Three novel, simple and reproducible high-performance liquid chromatography quantitative assays with UV detection were developed and validated for three major kavalactones--desmethoxyyangonin, methysticin and kavain--in rat liver microsomes using diazepam as an internal standard; liquid-liquid extraction was used for sample preparation and analysis was performed on a Shimadzu 10A high-performance liquid chromatography system. The analysis was carried out in reversed-phase mode with a Luna C(18) column (150 x 2.00 mm, 3 microm) at 40 degrees C. The limit of quantitation was 0.1 microg/mL using 0.25 mL of microsomal solution. The assays were linear over the range 0.1-10 microg/mL for desmethoxyyangonin, methysticin and kavain. Quality control samples exhibited good accuracy and precision with relative standard deviations lower than 15% and recoveries between 85 and 105%. The assays exhibited satisfactory performance with high sensitivity for quantifying desmethoxyyangonin, methysticin and kavain in rat liver microsomes and were successfully used to determine the three kavalactones and their microsomal metabolites.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP15345898 Desmethoxyyangonin Desmethoxyyangonin 15345-89-8 Price
qrcode