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High-performance Liquid Chromatography of Long Chain Aliphatic Aldehydes With Peroxyoxalate Chemiluminescence Detection Utilizing a Fluorogenic Reagent, 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole

High-performance Liquid Chromatography of Long Chain Aliphatic Aldehydes With Peroxyoxalate Chemiluminescence Detection Utilizing a Fluorogenic Reagent, 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole

K Nakashima, T Yoshida, N Kuroda, S Akiyama

Biomed Chromatogr. Mar-Apr 1996;10(2):99-101.

PMID: 8924737

Abstract:

A peroxyoxalate chemiluminescence detection in high-performance liquid chromatography was examined for determination of long chain aliphatic aldehydes using 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H) as a fluorogenic labelling reagent. A mixture of 0.6 mM bis(2,4,6-trichlorophenyl)oxalate and 100 mM H2O2 in acetonitrile was used as a post-column chemiluminescent reagent. DBD-hydrazones of five long chain aliphatic aldehydes (C11-C18:1) were eluted and well separated within 16 min with a mobile phase of 5 mM imidazole in acetonitrile. The detection limits were 14-18 fmol at a signal-to-noise ratio (S/N) of 2. DBD-hydrazones of four aliphatic aldehydes (C6-C11) were also eluted and well separated within 30 min with a mobile phase of 5mM imidazole buffer (pH 7.5)-acetonitrile (20/80, v/v). The detection limits were 86-152 fmol (S/N = 2).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP75203519	Bis(2-carbopentyloxy-3,5,6-trichlorophenyl) oxalate		75203-51-9	Price

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