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High-resolution Mapping of Nucleoprotein Complexes by Site-Specific protein-DNA Photocrosslinking: Organization of the Human TBP-TFIIA-TFIIB-DNA Quaternary Complex

High-resolution Mapping of Nucleoprotein Complexes by Site-Specific protein-DNA Photocrosslinking: Organization of the Human TBP-TFIIA-TFIIB-DNA Quaternary Complex

T Lagrange, T K Kim, G Orphanides, Y W Ebright, R H Ebright, D Reinberg

Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10620-5.

PMID: 8855228

Abstract:

We have used a novel site-specific protein-DNA photocrosslinking procedure to define the positions of polypeptide chains relative to promoter DNA in binary, ternary, and quaternary complexes containing human TATA-binding protein, human or yeast transcription factor IIA (TFIIA), human transcription factor IIB (TFIIB), and promoter DNA. The results indicate that TFIIA and TFIIB make more extensive interactions with promoter DNA than previously anticipated. TATA-binding protein, TFIIA, and TFIIB surround promoter DNA for two turns of DNA helix and thus may form a "cylindrical clamp" effectively topologically linked to promoter DNA. Our results have implications for the energetics, DNA-sequence-specificity, and pathway of assembly of eukaryotic transcription complexes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248156	TBP (TATA box binding protein) human			Price

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