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High Sensitivity and Non-Background SERS Detection of Endogenous Hydrogen Sulfide in Living Cells Using Core-Shell Nanoparticles

High Sensitivity and Non-Background SERS Detection of Endogenous Hydrogen Sulfide in Living Cells Using Core-Shell Nanoparticles

Wen-Shu Zhang, Ya-Ning Wang, Zhang-Run Xu

Anal Chim Acta. 2020 Jan 15;1094:106-112.

PMID: 31761035

Abstract:

Endogenous hydrogen sulfide (H2S) exists in multiple physiological processes. In order to further understand the action mechanism of H2S in cells and human body, we proposed a smart surface-enhanced Raman scattering (SERS) nanoprobe, Au core-4-mercaptobenzonitrile-Ag shell nanoparticle (Au@4-MBN@Ag), for the detection of endogenous H2S in living cells based on the reaction between Ag shell and sulfide species. 4-MBN was selected as the SERS reporter to avoid interference from cellular molecules. With the sulfide concentration increasing, the Ag2S constantly formed, and consequently the SERS signal intensity of Au@4-MBN@Ag gradually decreased owing to the weaker SERS activity of Ag2S. With the nanoprobes, this method not only offers a high sensitivity for H2S detection at an nM level, but also achieves the goal of non-background analysis. It displays satisfactory anti-interference capability and a good linear relationship with sulfide concentration ranging from 50 nM to 500 μM, and an estimated detection limit is 0.14 nM. The Au@4-MBN@Ag nanoprobes were successfully applied to detect endogenous H2S in living HepG2 cells stimulated by pyridoxal 5-phosphate monohydrate. This work offers a potential analytical method in the related research of H2S physiological function.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP41468251	Pyridoxal 5′-phosphate monohydrate		41468-25-1	Price

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