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High-throughput Plasmid Mini Preparations Facilitated by Micro-Mixing

High-throughput Plasmid Mini Preparations Facilitated by Micro-Mixing

W L Ng, M Schummer, F D Cirisano, R L Baldwin, B Y Karlan, L Hood

Nucleic Acids Res. 1996 Dec 15;24(24):5045-7.

PMID: 9016678

Abstract:

We have developed a reliable high-throughput plasmid isolation system using a 96-well plate format. This system combines a novel glass bead micro-mixing method with modified alkaline lysis and Sephacryl S-500 DNA purification procedures. Mechanical forces generated by vortexing glass beads inside each well of the 96-well plates ensure that the bacterial pellets are homogeneously resuspended, the cells are completely lyzed, and the resulting bacterial lysates are thoroughly mixed with the potassium acetate solution. The vortexing speed and duration for glass bead mixing have been standardized to facilitate plasmid DNA yields without significant adjustments.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP127082-A	Potassium acetate solution		127-08-2	Price

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