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Highly Selective Enrichment of Phosphopeptides by Titanium (IV) Attached Monodisperse-Porous Poly(vinylphosphonic Acid-Co-Ethylene Dimethacrylate) Microspheres

Highly Selective Enrichment of Phosphopeptides by Titanium (IV) Attached Monodisperse-Porous Poly(vinylphosphonic Acid-Co-Ethylene Dimethacrylate) Microspheres

Kouroush Salimi, Duygu Deniz Usta, Ömür Çelikbıçak, Aslı Pınar, Bekir Salih, Ali Tuncel

J Chromatogr A. 2017 May 5;1496:9-19.

PMID: 28351536

Abstract:

A seeded polymerization protocol was developed for the synthesis of monodisperse-porous poly(vinylphosphonic acid-co-ethylene dimethacrylate), [poly(VPA-co-EDMA)] microspheres with superior porous properties. The protocol allowed the direct synthesis of phosphonic acid functionalized porous microspheres with the mean size of ∼4μm and the specific surface area of 420m2g-1 without applying any complicated post-derivatization protocol for the attachment of phosphonic acid group. The phosphonic acid content of poly(VPA-co-EDMA) microspheres was determined as 1.5mmol H2PO3g-1 microspheres. Ti(IV) ions were attached onto the microspheres via metal-chelate complex formation by phosphonate-groups and Ti(IV) carrying monodisperse-porous poly(vinylphosphonic acid-co-ethylene dimethacrylate), [Ti(IV)@poly(VPA-co-EDMA)] microspheres were obtained as a new sorbent for phosphopeptide enrichment via immobilized metal affinity chromatography. The phosphopeptides in the enriched samples were identified by matrix-assited laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Four different phosphopeptides were detected with extremely high intensity by the treatment of β-casein digest prepared with the concentration of 10 fmol/mL with Ti(IV)@poly(VPA-co-EDMA) microspheres. Highly selective enrichment of phosphopeptides was also successfully carried out even at trace amounts in a complex mixture of digested proteins (molar ratio of β-casein to BSA, 1:1000) and eight different phosphorylated peptides from BSA digest were successfully identified. Moreover, four highly intense signals of the phosphopeptides in human serum were observed with high S/N ratio and clear background after enrichment by using Ti(IV)@poly(VPA-co-EDMA) microspheres.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP27754990	Poly(vinylphosphonic acid)		27754-99-0	Price

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