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Highly Selective Hydrolysis for the Outer Glucose at the C-20 Position in Ginsenosides by β-glucosidase From Thermus Thermophilus and Its Application to the Production of Ginsenoside F2 From Gypenoside XVII

Kyung-Chul Shin, Min-Ju Seo, Hye-Jin Oh, Deok-Kun Oh

Biotechnol Lett. 2014 Jun;36(6):1287-93.

PMID: 24563303

Abstract:

β-Glucosidase from Thermus thermophilus has specific hydrolytic activity for the outer glucose at the C-20 position in protopanaxadiol-type ginsenosides without hydrolysis of the inner glucose. The hydrolytic activity of the enzyme for gypenoside XVII was optimal at pH 6.5 and 90 °C, with a half-life of 1 h with 3 g enzyme l(-1) and 4 g gypenoside XVII l(-1). Under the optimized conditions, the enzyme converted the substrate gypenoside XVII to ginsenoside F2 with a molar yield of 100 % and a productivity of 4 g l(-1) h(-1). The conversion yield and productivity of ginsenoside F2 are the highest reported thus far among enzymatic transformations.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP80321693 Gypenoside XVII Gypenoside XVII 80321-69-3 Price
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