Home
Resources
Publications
Highly Sensitive SERS Detection and Quantification of Sialic Acid on Single Cell Using Photonic-Crystal Fiber With Gold Nanoparticles

Highly Sensitive SERS Detection and Quantification of Sialic Acid on Single Cell Using Photonic-Crystal Fiber With Gold Nanoparticles

Tianxun Gong, Ying Cui, Douglas Goh, Kong Kien Voon, Perry Ping Shum, Georges Humbert, Jean-Louis Auguste, Xuan-Quyen Dinh, Ken-Tye Yong, Malini Olivo

Biosens Bioelectron. 2015 Feb 15;64:227-33.

PMID: 25222325

Abstract:

An ultrasensitive surface enhanced Raman spectroscopy (SERS) based sensing platform was developed to detect the mean sialic acid level on the surface of single cell with sensitivity as low as 2 fmol. This platform adopted the use of an interference-free Raman tag, 4-(dihydroxyborophenyl) acetylene (DBA), which selectively binds to sialic acid on the cell membrane. By loading the side channel of a photonic crystal fiber with a mixture of gold nanoparticles and DBA-tagged HeLa cell, and subsequently propagating laser light through the central solid core, strong SERS signal was obtained. This SERS technique achieved accurate detection and quantification of concentration of sialic acid on a single cell, surpassing previously reported methods that required more than 10(5) cells. Moreover, this platform can be developed into a clinical diagnostic tool to potentially analyze sialic acid-related diseases such as tumor malignancy and metastasis in real-time.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS793950	4-(dihydroxyborophenyl)acetylene			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: