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Highly Stable Lyophilized Homogeneous Bead-Based Immunoassays for On-Site Detection of Bio Warfare Agents From Complex Matrices

Adva Mechaly, Sharon Marx, Orly Levy, Shmuel Yitzhaki, Morly Fisher

Anal Chem. 2016 Jun 21;88(12):6283-91.

PMID: 27253489

Abstract:

This study shows the development of dry, highly stable immunoassays for the detection of bio warfare agents in complex matrices. Thermal stability was achieved by the lyophilization of the complete, homogeneous, bead-based immunoassay in a special stabilizing buffer, resulting in a ready-to-use, simple assay, which exhibited long shelf and high-temperature endurance (up to 1 week at 100 °C). The developed methodology was successfully implemented for the preservation of time-resolved fluorescence, Alexa-fluorophores, and horse radish peroxidase-based bead assays, enabling multiplexed detection. The multiplexed assay was successfully implemented for the detection of Bacillus anthracis, botulinum B, and tularemia in complex matrices.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42411217 Peroxidase Stabilizing Buffer Peroxidase Stabilizing Buffer Price
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