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Histological Localization of Nervous-System Antigens in the Cerebellum by Immunoperoxidase Labeling

Histological Localization of Nervous-System Antigens in the Cerebellum by Immunoperoxidase Labeling

M Schachner, M Z Ruberg, T B Carnow

Brain Res Bull. Jul-Aug 1976;1(4):367-77.

PMID: 61794

Abstract:

The indirect immunoperoxidase method was used to localize histologically on sagittal sections of mouse cerebellum antigenic determinants detected by the following antisera: anti-NS-2, anti-NS-3, anti-NS-4 rabbit anti-bovine corpus callosum, rabbit anti-mouse brain, rabbit anti-glial fibrillary acidic protein, and rabbit anti-neurofilament protein. Anti-alpha-bungarotoxin serum and normal rabbit serum were used as negative controls. The various sera showed similarities in staining pattern as well as differences. Anti-NS-2 antiserum labeled the somata of interneurons in the molecular layer, granule cell bodies, glial cells in the white matter, and along the surfaces of blood vessels. A similar pattern of staining is produced by the anti-NS-3 antiserum except that glial cells are less prominent in the white matter and the blood vessels are not visible at all. Anti-NS-4 antiserum does not label interneurons but does label glomeruli and, less intensely, granule cell bodies in the granular layer. Rabbit anti-mouse brain antiserum is similar to anti-NS-4 antiserum except that fiber tracts in the white matter are stained more intensely; Rabbit anti-bovine corpus callosum labels only white matter. Antisera to neurofilament ans astrocytes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4247689	Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit			Price

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