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HPLC Analysis of Fatty Acyl-Glycine in the Aqueous Methanesulfonic Acid Hydrolysates of N-terminally Fatty Acylated Peptides

HPLC Analysis of Fatty Acyl-Glycine in the Aqueous Methanesulfonic Acid Hydrolysates of N-terminally Fatty Acylated Peptides

Keiko Okimura, Kazuhiro Ohki, Sotoo Nagai, Naoki Sakura

Biol Pharm Bull. 2003 Aug;26(8):1166-9.

PMID: 12913269

Abstract:

Acylation with long-chain fatty acids is a common modification at the N-terminal glycine residues of natural proteins. In this work, we performed HPLC analysis of myristoylglycine (Myr-Gly-OH), palmitoylglycine (Pal-Gly-OH) or lauroylglycine (Lau-Gly-OH), which were produced in the hydrolysates of synthetic Myr-Gly-, Pal-Gly-, or Lau-Gly-peptides, respectively, by means of a mild acid hydrolysis in methanesulfonic acid : dioxane : water (2 : 1 : 1) at 60 degrees C for 12 h. Myr-Gly-OH, Pal-Gly-OH and Lau-Gly-OH were quite stable under hydrolysis conditions. These fatty acyl-Gly-OH were conveniently detectable at a 20 nmol level by direct reversed-phase HPLC. Thus, mild acid hydrolysis, followed by HPLC analysis of the hydrolysate, provides a simple method of identification of the N-terminal structure of fatty acyl-Gly-peptides.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2441410	PalGly		2441-41-0	Price

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