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HPLC Method for the Determination of Rosiglitazone in Human Plasma and Its Application in a Clinical Pharmacokinetic Study

HPLC Method for the Determination of Rosiglitazone in Human Plasma and Its Application in a Clinical Pharmacokinetic Study

Rao N V S Mamidi, Madhusudana R Chaluvadi, Biju Benjamin, Mullangi Ramesh, Kasiram Katneni, Aravinda P Babu, Jaydip Bhanduri, Naidu M U Rao, Ramanujam Rajagopalan

Arzneimittelforschung. 2002;52(7):560-4.

PMID: 12189780

Abstract:

Rosiglitazone (CAS 155141-29-0, Avandia) is a novel insulin sensitizer used in the treatment of type 2 diabetes. A sensitive high performance liquid chromatography (HPLC) method for its determination in human plasma using fluorescence detection (excitation: 247 nm, emission: 367 nm) with a suitable internal standard (I. S.) is described. Ethyl acetate was used as extraction solvent. A mobile phase consisting of phosphate buffer, acetonitrile and methanol was used at a flow rate of 1.0 ml/min on a C18 column. The absolute recovery was > 90% and the lower limit of quantitation was 5 ng/ml. The intra- and inter-day relative standard deviations ranged from 0.58-6.69% and 0.82-6.63%, respectively. The method described is simple, economical, precise and accurate and has been successfully applied in a pharmacokinetic study conducted in healthy human volunteers.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP155141290	Rosiglitazone - CAS 155141-29-0		155141-29-0	Price

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