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HPLC/PDA Determination of Carminic Acid and 4-aminocarminic Acid Using Relative Molar Sensitivities With Respect to Caffeine

HPLC/PDA Determination of Carminic Acid and 4-aminocarminic Acid Using Relative Molar Sensitivities With Respect to Caffeine

Yuzo Nishizaki, Naoko Sato-Masumoto, Aki Yokota, Tsuyoshi Mikawa, Koichi Nakashima, Taichi Yamazaki, Miho Kuroe, Masahiko Numata, Toshihide Ihara, Yusai Ito, Naoki Sugimoto, Kyoko Sato

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2018 May;35(5):838-847.

PMID: 29447580

Abstract:

To accurately determine carminic acid (CA) and its derivative 4-aminocarminic acid (4-ACA), a novel, high-performance liquid chromatography with photodiode array detector (HPLC/PDA) method using relative molar sensitivity (RMS) was developed. The method requires no analytical standards of CA and 4-ACA; instead it uses the RMS values with respect to caffeine (CAF), which is used as an internal standard. An off-line combination of 1H-quantitative nuclear magnetic resonance spectroscopy (1H-qNMR) and HPLC/PDA was able to precisely determine the RMSs of CA274nm/CAF274nm and 4-ACA274nm/CAF274nm. To confirm the performance of the HPLC/PDA method using RMSs, the CA and 4-ACA contents in test samples were tested using four different HPLC-PDA instruments and one HPLC-UV. The relative standard deviations of the results obtained from five chromatographs and two columns were less than 2.7% for CA274nm/CAF274nm and 1.1% for 4-ACA274nm/CAF274nm. The 1H-qNMR method was directly employed to analyse the CA and 4-ACA contents in test samples. The differences between the quantitative values obtained from both methods were less than 5% for CA and 3% for 4-ACA. These results demonstrate that the HPLC/PDA method using RMSs to CAF is a simple and reliable quantification method that does not require CA and 4-ACA certified reference materials.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1260179	Carminic acid		1260-17-9	Price

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