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HPLC/tandem Mass Spectrometric Studies on Steroidal Saponins: An Example of Quantitative Determination of Shatavarin IV From Dietary Supplements Containing Asparagus Racemosus

HPLC/tandem Mass Spectrometric Studies on Steroidal Saponins: An Example of Quantitative Determination of Shatavarin IV From Dietary Supplements Containing Asparagus Racemosus

Dada Patil, Manish Gautam, Sunil Gairola, Suresh Jadhav, Bhushan Patwardhan

J AOAC Int. Nov-Dec 2014;97(6):1497-502.

PMID: 25632427

Abstract:

Asparagus racemosus (AR) is a popular botanical present in several Ayurvedic medicines and nutritional and dietary supplements with immunomodulatory, galactogogue, and anticancer activity. A steroidal saponin known as shatavarin IV is one of the active constituents of AR. A new, selective, and rapid HPLC/MSIMS method has been developed and validated for quantitative estimation of shatavarin IV in crude, processed, and marketed samples of AR. The analytes were separated on a Luna C18 column using simple isocratic elution with water (0.1% acetic acid)-acetonitrie;(0.1% acetic acid; 70 + 30, vIv) at a flow rate of 0.8 mLlmin. The analytes were detected by electrospray ionization (ESI)-MS/MS and quantified using multiple reaction monitoring techniques in the positive ion mode. The method showed excellent linearity (r2 > 0.998) over the concentration range of 7.5 to 254 ng/mL with LOD of 2.5 ng/mL. Precision (RSD) and accuracy (recovery) were found in the ranges of 2.00 to 5.15 and 102 to 110%, respectively. The validated HPLC/ESI-MS/MS method was successfully applied to the quantification of shatavarin IV in crude, processed, and marketed (single or multiherb) AR samples. Therefore, this method could be used for QC and standardization of pharmaceutical or nutritional products containing AR.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP84633341	Shatavarin IV		84633-34-1	Price

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