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Human interleukin-2 (IL-2) Expressed by Transfected Mammalian Cells

Human interleukin-2 (IL-2) Expressed by Transfected Mammalian Cells

M Knezević, K Vorauer-Uhl, O Hohenwarter, F Steindl, R Grabherr, P Raspor

Pflugers Arch. 1996;431(6 Suppl 2):R227-8.

PMID: 8739347

Abstract:

cDNA for human interleukin-2 (IL-2) was cloned into the pRc/RSV vector for expression in animal cells. Baby hamster kidney (BHK-21) cells and Chinese hamster ovary (CHO) cells were transfected several times using calcium phosphate and electroporation methods with the construct pRc/RSV SIGIL2. Different transfection efficiencies were obtained. The biological test on CTLL-2 (mouse cytotoxic lymphocytes) showed that the kinetics of cell proliferation were different from those of rIL-2 (recombinant IL-2) expressed in bacteria and in BHK cells. When high concentrations of rIL-2 were applied, an inhibitory effect on CTLL-2 was observed when bacterial product was used, whilst rBHK interleukin caused no inhibition. Recombinant BHK IL2 induced a slower response of CTLL-2 cells at the beginning of the cultivation, however, prolonged activity was detected at the later stage of the experiment.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248755	IL-2 human			Price

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