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Human Monoclonal Anti-Idiotypic Antibody to the Tumour-Associated Antibody 791T/36

Human Monoclonal Anti-Idiotypic Antibody to the Tumour-Associated Antibody 791T/36

E B Austin, R A Robins, L G Durrant, M R Price, R W Baldwin

Immunology. 1989 Aug;67(4):525-30.

PMID: 2788611

Abstract:

A human monoclonal antibody, 105AD7, was produced by fusion of a mouse/human heteromyeloma cell line with lymphocytes from a patient previously injected with mouse monoclonal antibody 791T/36 for tumour immunoscintigraphy. The 105AD7 hybridoma has been in continuous culture for more than 12 months, producing a human monoclonal IgG1 which binds to 791T/36 (IgG2b) and its IgG2a class switch variant, but not a range of other monoclonal mouse immunoglobulins. In quantitative flow cytometric assays, 105AD7 was shown to block the binding of fluorescein-labelled 791T/36 to its target gp72 antigen at the surface of tumour cells, but not the binding of 228, an anti-carcinoembryonic antigen (CEA) monoclonal antibody to CEA. Tests with purified 105AD7 antibody demonstrated a stoichiometric high-affinity interaction between 105AD7 and 791T/36. Thus 105AD7 is a human anti-idiotypic antibody which binds at or very close to the binding site of 791T/36, and as such is a candidate for anti-idiotypic immunization of cancer patients.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414841	Monoclonal Anti-Carcinoembryonic Antigen antibody produced in mouse			Price

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