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Human Plasma Protein N-glycosylation

Human Plasma Protein N-glycosylation

Florent Clerc, Karli R Reiding, Bas C Jansen, Guinevere S M Kammeijer, Albert Bondt, Manfred Wuhrer

Glycoconj J. 2016 Jun;33(3):309-43.

PMID: 26555091

Abstract:

Glycosylation is the most abundant and complex protein modification, and can have a profound structural and functional effect on the conjugate. The oligosaccharide fraction is recognized to be involved in multiple biological processes, and to affect proteins physical properties, and has consequentially been labeled a critical quality attribute of biopharmaceuticals. Additionally, due to recent advances in analytical methods and analysis software, glycosylation is targeted in the search for disease biomarkers for early diagnosis and patient stratification. Biofluids such as saliva, serum or plasma are of great use in this regard, as they are easily accessible and can provide relevant glycosylation information. Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. In addition, the less abundant immunoglobulins D and E are included because of their major relevance in immunology and biopharmaceutical research. Where available, the glycosylation is described in a site-specific manner. In the discussion, we put the glycosylation of individual proteins into perspective and speculate how the individual proteins may contribute to a total plasma N-glycosylation profile determined at the released glycan level.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP9013712	Hemopexin from human plasma	9013-71-2	Price
AP90170802-B	Antithrombin III from human plasma	90170-80-2	Price
IAR42410741	α2-hs-Glycoprotein from human plasma		Price
IAR4247132	Apolipoprotein B from human plasma		Price

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