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Hybrid Glycophorin and Red Blood Cell Antigen Genotyping in Asian American Type O Blood Donors With Mi

Hybrid Glycophorin and Red Blood Cell Antigen Genotyping in Asian American Type O Blood Donors With Mi

Xin Lin, Graciela Rubio, Jigar Patel, Sukanta Banerjee, Tom Frame, Norkeshia Billups, Jiacheng Yang

Transfusion. 2019 Dec;59(12):3767-3775.

PMID: 31697429

Abstract:

Background:
The GP.Mur glycophorin with Mia phenotype is relatively common and clinically significant in the Southeast Asian populations. The aim of this study is to genotype Mia -positive Asian American type O blood donors. Red blood cell (RBC) minor antigens were also determined in the same cohort.
Study design and methods:
Asian American blood donors of the Gulf Coast Regional Blood Center (Houston, TX) were screened using a typing reagent (NOVACLONE Anti-Mia Monoclonal IgG Typing Reagent, Dominion Biologicals Ltd) from March 2016 to July 2018. Aliquots of Mia -positive blood from type O donors were subjected to serologic confirmation using Mia - and/or Mur-specific GAMA210 and 64D6 monoclonal antibodies, and two human antisera. Extracted genomic DNA was amplified by polymerase chain reaction (PCR) using GYP hybrid gene/allele-specific primers followed by bidirectional Sanger sequencing. Zygosity for GYP*Mur and GYP*Bun was determined using TaqMan real-time PCR assay. Phenotypes of 35 RBC antigens and three phenotypic variants were determined with use of an in vitro diagnostic test, PreciseType HEA Molecular BeadChip Test (Immucor).
Results:
By screening 4600 blood donations in the Houston metropolitan area, 209 samples from 103 unique donors were identified to be Mia -positive. By PCR and sequencing analysis, 97 of the 103 Mia -positive donors carried hybrid genes GYP*Mur (89.7% including two homozygotes), GYP*Bun (6.2%), GYP*Vw (3.1%) and GYP*Hut (1.0%). Concordance between serology and DNA analysis was 98%, 99%, and 100% for the GAMA210, 64D6, and human antisera, respectively. Genotyping of RBC antigens showed that the Mia -positive donors were predominantly associated M+ N- S- s+ (48.5%) and M+ N+ S- s+ (38.1%) phenotypes.
Conclusions:
The GP.Mur glycophorin is most prevalent in the Mia -positive Asian American type O blood donors.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP76416154-B	Glycophorin Predominantly glycophorin A from blood type MN		76416-15-4	Price

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