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Hydrogen Production by Fermentation Using Acetic Acid and Lactic Acid

Mitsufumi Matsumoto, Yasuhiko Nishimura

J Biosci Bioeng. 2007 Mar;103(3):236-41.

PMID: 17434426

Abstract:

Microbial hydrogen production from sho-chu post-distillation slurry solution (slurry solution) containing large amounts of organic acids was investigated. The highest hydrogen producer, Clostridium diolis JPCC H-3, was isolated from natural environment and produced hydrogen at 6.03+/-0.15 ml from 5 ml slurry solution in 30 h. Interestingly, the concentration of acetic acid and lactic acid in the slurry solution decreased during hydrogen production. The substrates for hydrogen production by C. diolis JPCC H-3, in particular organic acids, were investigated in an artificial medium. No hydrogen was produced from acetic acid, propionic acid, succinic acid, or citric acid on their own. Hydrogen and butyric acid were produced from a mixture of acetic acid and lactic acid, showing that C. diolis. JPCC H-3 could produce hydrogen from acetic acid and lactic acid. Furthermore, calculation of the Gibbs free energy strongly suggests that this reaction would proceed. In this paper, we describe for the first time microbial hydrogen production from acetic acid and lactic acid by fermentation.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP233759983 Fluorescein-O′-acetic acid Fluorescein-O′-acetic acid 233759-98-3 Price
IAR4244979 STO-609-acetic acid STO-609-acetic acid Price
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