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Hydrolase-catalyzed Beta-Mannosylations

N Taubken, J Thiem

Glycoconj J. 1998 Aug;15(8):757-67.

PMID: 9870351

Abstract:

Transmannosylations catalysed by beta-mannosidase from snail viscera or beta-galactosidase from Aspergillus oryzae were accomplished with 4-nitrophenyl beta-D-mannopyranoside as donor substrate. With suitable hydrophobic acceptor molecules preferentially beta1-4-linked disaccharides were obtained. The activities of both glycosidases in buffer cosolvent mixtures were determined, and conditions for their immobilization were elaborated and optimized. A model of the enzymic transfer mechanism is suggested.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP35599021 4-Nitrophenyl β-D-mannopyranoside 4-Nitrophenyl β-D-mannopyranoside 35599-02-1 Price
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