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Hydrolysis of Glyceryl tri(1- 14 C)octanoate and Glyceryl tri(1- 14 C)oleate Monolayers by Postheparin Lipolytic Activity

Hydrolysis of Glyceryl tri(1- 14 C)octanoate and Glyceryl tri(1- 14 C)oleate Monolayers by Postheparin Lipolytic Activity

L C Smith

J Lipid Res. 1972 Nov;13(6):769-76.

PMID: 4641417

Abstract:

The hydrolysis of monomolecular films of glyceryl tri[1-(14)C]octanoate and glyceryl tri[1-(14)C]oleate has been demonstrated by measurement of the decrease in surface radio-activity that occurs in the presence of postheparin plasma. The hydrolysis displayed first order kinetics and was proportional to enzyme concentration over a 10-fold range. No hydrolysis was observed in the absence of enzyme, and only slight activity (1%) was found in plasma taken from subjects before heparin administration. The hydrolysis was stimulated to a variable extent by Ca(2+). The first product of hydrolysis of the monolayer was identified as 1,2-diglyceride, which was subsequently converted to 2-monoglyceride. Inhibition of triglyceride hydrolysis was observed when postheparin plasma was preincubated in 2 m NaCl, 10(-4) m protamine, 10 mm Na(4)P(2)O(7), and 0.1 m NaF. Monolayer techniques avoid some but not all of the problems associated with emulsified lipid substrates and appear to be applicable for study of post-heparin lipolytic activities.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP168294573	Glyceryl tri(palmitate-1-13C)		168294-57-3	Price

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