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Hydrolysis of Mixed Monomolecular Films of phosphatidylcholine/triacylglycerol by Pancreatic Phospholipase A2

G Pieroni, R Verger

Eur J Biochem. 1983 May 16;132(3):639-44.

PMID: 6852018

Abstract:

We studied the effect of glycerides on pancreatic phospholipase A2 hydrolysis of mixed monomolecular films of trioctanoylglycerol/1,2-didodecanoyl-sn-glycero-3-phosphocholine with the technique of Piéroni and Verger [(1979) J. Biol. Chem. 254, 10090-10094]. The quantity of enzyme adsorbed to the interface was concomitantly determined with [3H]amidinated phospholipase. At phospholipid packing above the critical penetration pressure, triacylglycerol stimulates phosphatidylcholine hydrolysis to a great extent. On the other hand, the activity of pancreatic phospholipase A2 on a mixed film is inhibited by the action of pancreatic lipase. Interface binding of phospholipase A2 to the lipid substrate does not imply activity.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP18194257 1,2-Didodecanoyl-sn-glycero-3-phosphocholine 1,2-Didodecanoyl-sn-glycero-3-phosphocholine 18194-25-7 Price
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