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[Hydrolysis of N-acetyl-L- And N-acetyl-D,L-methionine by Microbial Aminacylase]

[Hydrolysis of N-acetyl-L- And N-acetyl-D,L-methionine by Microbial Aminacylase]

I A Iamskov, M V Budanov, L S Lobareva, M K Malinka, V A Davankov

Biokhimiia. 1981 Apr;46(4):652-6.

PMID: 7284482

Abstract:

The kinetics of N-acetyl-L- and N-acetyl-D,L-methionine hydrolysis by aminoacylase from Asp. oryzae were studied. The maximal rate of the reaction was found to be about 2000 mu moles of L-methionine per mg of protein per hour; Km was equal to about 1.10(-1) M for both the racemic and optically active substrates. In the presence of Co(II) ions (the molar ratio of N-acetyl-L-methionine/Co(II) was 100:1) the reaction velocity was increased. The values of approximately 4500 and approximately 3000 mu moles per mg per hour were-attained with L-enantiomer and racemic substrates, respectively. The Km values were decreased down to 2.10(-2) M and 1.4.10(-2) M, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1115475	N-Acetyl-D,L-Methionine		1115-47-5	Price

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