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Hydropyrolysis Over a Platinum Catalyst as a Preparative Technique for the Compound-Specific Carbon Isotope Ratio Measurement of C27 Steroids

Hydropyrolysis Over a Platinum Catalyst as a Preparative Technique for the Compound-Specific Carbon Isotope Ratio Measurement of C27 Steroids

Will Meredith, Rachel L Gomes, Mick Cooper, Colin E Snape, Mark A Sephton

Rapid Commun Mass Spectrom. 2010 Mar 15;24(5):501-5.

PMID: 20112272

Abstract:

Compound-specific stable carbon isotope analysis by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is an important method for the determination of the (13)C/(12)C ratios of biomolecules such as steroids, for a wide range of applications. However, steroids in their natural form exhibit poor chromatographic resolution, while derivatisation adds carbon thereby corrupting the stable isotopic composition. Hydropyrolysis with a sulphided molybdenum catalyst has been shown to defunctionalise the steroids, while leaving their carbon skeleton intact, allowing for the accurate measurement of carbon isotope ratios. The presence of double bonds in unsaturated steroids such as cholesterol resulted in significant rearrangement of the products, but replacing the original catalyst system with one of platinum results in higher conversions and far greater selectivity. The improved chromatographic performance of the products should allow GC/C/IRMS to be applied to more structurally complex steroid hormones and their metabolites.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AS21221	Platinum (isotope ratio)			Price

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