Home
Resources
Publications
Hypersensitivity of Pulmonary Chemoreflex Induced by poly-L-lysine: Role of Cationic Charge

Hypersensitivity of Pulmonary Chemoreflex Induced by poly-L-lysine: Role of Cationic Charge

Qihai Gu, Ruei-Lung Lin, Thomas C Vanaman, Lu-Yuan Lee

Respir Physiol Neurobiol. 2006 Mar 28;151(1):31-43.

PMID: 15996907

Abstract:

This study was carried out to investigate the role of cationic charge in the hypersensitivity of pulmonary C-fibers induced by airway exposure to synthetic cationic protein poly-L-lysine (PLL) in anesthetized rats. Inhalation of PLL aerosol induced a distinctly irregular breathing pattern, and significantly enhanced the pulmonary chemoreflex responses to capsaicin. However, after the cationic charges were completely removed from PLL by succinylation, the succinylated PLL no longer produced any change in either the baseline breathing pattern or the reflex responses to capsaicin. In addition, the effects of PLL were also abolished after premixing it with a polyanion, poly-L-glutamic or poly-L-aspartic acid, before delivery. In sharp contrast, when delivered within 5 min after the PLL aerosol, these two polyanions were completely ineffective in reversing the effects of PLL. Electrophysiological recording of the afferent activity of single pulmonary C-fibers further supported our conclusion that the cationic charge carried by this protein is primarily responsible for generating the stimulatory and sensitizing effects of PLL on these afferents.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS741220	Poly-L-lysine, succinylated			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: