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Identification and Characterization of D-Arabinose Reductase and D-Arabinose Transporters From Pichia Stipitis

Identification and Characterization of D-Arabinose Reductase and D-Arabinose Transporters From Pichia Stipitis

Seiya Watanabe, Yuki Utsumi, Shigeki Sawayama, Yasuo Watanabe

Biosci Biotechnol Biochem. 2016 Nov;80(11):2151-2158.

PMID: 27400037

Abstract:

d-xylose and l-arabinose are the major constituents of plant lignocelluloses, and the related fungal metabolic pathways have been extensively examined. Although Pichia stipitis CBS 6054 grows using d-arabinose as the sole carbon source, the hypothetical pathway has not yet been clarified at the molecular level. We herein purified NAD(P)H-dependent d-arabinose reductase from cells grown on d-arabinose, and found that the enzyme was identical to the known d-xylose reductase (XR). The enzyme activity of XR with d-arabinose was previously reported to be only 1% that with d-xylose. The kcat/Km value with d-arabinose (1.27 min-1 mM-1), which was determined using the recombinant enzyme, was 13.6- and 10.5-fold lower than those with l-arabinose and d-xylose, respectively. Among the 34 putative sugar transporters from P. stipitis, only seven genes exhibited uptake ability not only for d-arabinose, but also for d-glucose and other pentose sugars including d-xylose and l-arabinose in Saccharomyces cerevisiae.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP10323203	D-(−)-Arabinose		10323-20-3	Price

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