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Identification and Characterization of EYD1, Encoding an Erythritol Dehydrogenase in Yarrowia Lipolytica and Its Application to Bioconvert Erythritol Into Erythrulose

Frédéric Carly, Sébastien Steels, Samuel Telek, Marie Vandermies, Jean-Marc Nicaud, Patrick Fickers

Bioresour Technol. 2018 Jan;247:963-969.

PMID: 30060436

Abstract:

In this study, gene YALI0F01650g has been isolated and characterized. Several experimental evidences suggest that the identified gene, renamed EYD1, encodes an erythritol dehydrogenase. An efficient bioreactor process for the bioconversion of erythritol into erythrulose was also developed. Using constitutive expression of EYD1 in a Y. lipolytica mutant containing a disrupted EYK1 gene, which encodes erythrulose kinase, erythrulose could be synthesized from erythritol at a rate of 0.116g/gDCW.h and with a bioconversion yield of 0.64g/g.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP533506 Erythrulose Erythrulose 533-50-6 Price
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