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Identification and Characterization of Receptor for Cytokine-Induced Neutrophil chemoattractant-3 on Rat Neutrophils

Identification and Characterization of Receptor for Cytokine-Induced Neutrophil chemoattractant-3 on Rat Neutrophils

K Murakami, F Shibata, M al-Mokdad, H Nakagawa, A Ueno, T Kondo

Biochem Biophys Res Commun. 1997 Mar 17;232(2):562-7.

PMID: 9125223

Abstract:

Cytokine-induced neutrophil chemoattractants (CINCs), members of alpha-chemokines, are considered to be major neutrophil chemotactic factors in rats. Recombinant CINC-3/rat macrophage inflammatory protein-2 (MIP-2) having an additional tyrosine residue at the carboxyl terminus (CINC-3-Tyr) was constructed, purified, radiolabeled with 125I, and used for binding studies. The specific binding of 125I-labeled CINC-3-Tyr (125I-CINC-3-Tyr) to rat neutrophils reached a plateau after approximately 60 min at 4 degrees C. This binding of 125I-CINC-3-Tyr could be reversed by adding an excess amount of unlabeled CINC-3-Tyr. Scatchard analysis revealed approximately 12,000 binding sites per cell on rat neutrophils with an apparent Kd value of 120 pM. Chemical cross-linking experiments suggested that the rat neutrophil CINC-3 receptor is a mass of approximately 69 kDa. Taken together, our results strongly suggest that rat neutrophils express a high affinity receptor for CINC-3.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413073	Cytokine-Induced Neutrophil Chemoattractant-3 from rat			Price

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