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Identification of 7-hydroperoxycholesterol in Human Liver by Liquid Chromatography-Mass Spectrometry

J Adachi, M Asano, Y Ueno, T Naito

Alcohol Clin Exp Res. 2000 Apr;24(4 Suppl):21S-25S.

PMID: 10803774

Abstract:

Background:
A liquid chromatography-mass spectrometry with an atmospheric chemical ionization interface has been developed to identify simultaneously various oxygenated cholesterols in lipid extracts from alcoholic fatty liver.
Methods:
We collected human liver tissues from alcoholic and control subjects at autopsy. We purified the lipid extracts with solid phase extraction and injected them onto a spherisorb ODS column, by using methanol that contained 10 mM ammonium acetate as a mobile phase and a flow rate of 0.7 ml/min.
Results:
We found the presence of 7-hydroperoxycholesterol and other oxysterols (5-cholesten-3beta-ol-7-one, cholest-5-ene-3beta, 7-diol, and 5, 6beta-epoxy-5beta-cholestan-3beta-ol) in alcoholic fatty liver tissues.
Conclusions:
We assume that long-term ethanol ingestion leads to excessive oxidative stress and to the accumulation of cholesterol-derived hydroperoxides and oxysterols in alcoholic fatty liver.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP566289 5-Cholesten-3β-ol-7-one 5-Cholesten-3β-ol-7-one 566-28-9 Price
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