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Identification of a Novel Esterase From Marine Environmental Genomic DNA Libraries and Its Application in Production of Free All- trans-Astaxanthin

Identification of a Novel Esterase From Marine Environmental Genomic DNA Libraries and Its Application in Production of Free All- trans-Astaxanthin

Ping Lu, Xinwei Gao, Hao Dong, Zhen Liu, Francesco Secundo, Changhu Xue, Xiangzhao Mao

J Agric Food Chem. 2018 Mar 21;66(11):2812-2821.

PMID: 29468878

Abstract:

Astaxanthin is a pigment with various functions. Free astaxanthin is obtained mainly through saponification methods, which could result in many byproducts. Enzymatic methods using lipases have been used in a few cases, while there are no reports on the use of esterases for the production of free astaxanthin. Herein we present the screening and identification of a novel esterase (Est3-14) from a marine mud metagenomic library. Est3-14 is pH-sensitive and keeps good stability in alkaline buffers (residual activity 94%, pH 8.0, 4 °C, and 36 h). Meanwhile, Est3-14 keeps a good stability in the medium temperature condition (residual activity 56.7%, pH 8.0, 40 °C, and 84 h). Est3-14 displayed high hydrolysis activity to prepare free all- trans-astaxanthin in biphasic systems. Furthermore, under optimal conditions (0.5 mL ethanol, 6 mL 0.1 M Tris-HCl buffer, pH 8.0, 0.5% (w/v) H. pluvialis oil, 40 °C), the hydrolytic conversion ratio was 99.3% after 36 h.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP472617-A	all-trans-Astaxanthin		472-61-7	Price

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