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Identification of an Elastolytic Protease in Stationary Phase Culture Filtrates of M. Tuberculosis

Identification of an Elastolytic Protease in Stationary Phase Culture Filtrates of M. Tuberculosis

S S Rowland, J L Ruckert, B N Burall Jr

FEMS Microbiol Lett. 1997 Jun 1;151(1):59-64.

PMID: 9198282

Abstract:

Culture filtrate from M. tuberculosis grown in Sauton broth was screened at weekly intervals for elastase and caseinase activity. Both activities were detected concomitantly at 4 weeks and had similar inhibitor and pH profiles. Highest activity occurred between pH 6.5 and pH 7.5. Azocaseinase activity was linear with time between 12 and 28 h at 37 degrees C. Enzymatic activity was inhibited by EDTA, EGTA, dithiothreitol, ethylmaleimide, 1,10-phenanthroline, Zincov, N-tosyl-L-phenylalanine chloromethyl ketone, and N-methoxysuccinyl-Ala-Ala-Pro-Val chloromethyl ketone. SDS-PAGE analysis revealed breakdown of casein after incubation with culture filtrate. These results indicate the presence of a calcium-dependent, elastolytic metalloprotease in stationary phase cultures of M. tuberculosis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP65144345	N-(Methoxysuccinyl)-Ala-Ala-Pro-Val-chloromethyl ketone		65144-34-5	Price

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