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Identification of Caerulomycin A Gene Cluster Implicates a Tailoring Amidohydrolase

Yiguang Zhu, Peng Fu, Qinheng Lin, Guangtao Zhang, Haibo Zhang, Sumei Li, Jianhua Ju, Weiming Zhu, Changsheng Zhang

Org Lett. 2012 Jun 1;14(11):2666-9.

PMID: 22591508

Abstract:

The biosynthetic gene cluster for caerulomycin A (1) was cloned and characterized from the marine actinomycete Actinoalloteichus cyanogriseus WH1-2216-6, which revealed an unusual hybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) system. The crmL disruption mutant accumulated caerulomycin L (2) with an extended L-leucine at C-7, implicating an amidohydrolase activity for CrmL. The leucine-removing activity was confirmed for crude CrmL enzymes. Heterologous expression of the 1 gene cluster led to 1 production in Streptomyces coelicolor.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP21802379 Caerulomycin A Caerulomycin A 21802-37-9 Price
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