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Identification of Ceramide-Phosphorylethanolamine in Oomycete Plant Pathogens: Pythium Ultimum, Phytophthora Infestans, and Phytophthora Capsici

Identification of Ceramide-Phosphorylethanolamine in Oomycete Plant Pathogens: Pythium Ultimum, Phytophthora Infestans, and Phytophthora Capsici

R A Moreau, D H Young, P O Danis, M J Powell, C J Quinn, K Beshah, R A Slawecki, R L Dilliplane

Lipids. 1998 Mar;33(3):307-17.

PMID: 9560806

Abstract:

Cellular lipids were extracted from three species of Oomycete plant pathogens (Pythium ultimum, Phytophthora infestans, and Ph. capsici) and analyzed via normal-phase high-performance liquid chromatography with flame-ionization detection. The most abundant polar lipids in each of the three species were the polar membrane lipids, phosphatidylethanolamine (PE), phosphatidylcholine, and a phosphosphingolipid that eluted soon after PE. Structural analysis via mass spectrometry and nuclear magnetic resonance spectrometry revealed that the phosphosphingolipid was ceramide phosphorylethanolamine (Cer-PE). The most abundant molecular species of Cer-PE in P. ultimum had a molecular weight of 670.5, contained an unusual 19-carbon branched triunsaturated sphingoid (C19-delta 4, 8, 10, 9-methyl long-chain base) and palmitic acid as the amide-linked fatty acid. The most abundant molecular species of Cer-PE in Ph. infestans had a molecular weight of 714.5, contained a common 16-carbon 1,3 di-OH sphingoid, and erucic (cis 13-docosenoic, C22-delta 13) acid as the amide-linked fatty acid. The Cer-PE in Ph. capsici comprised a mixture of each of the two molecular species found in P. ultimum and Ph. infestans.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4242652	Ceramide phosphorylethanolamine			Price

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