Home
Resources
Publications
Identification of Impurities in Erythromycin by Liquid Chromatography-Mass Spectrometric Detection

Identification of Impurities in Erythromycin by Liquid Chromatography-Mass Spectrometric Detection

Satish Kumar Chitneni, Cindy Govaerts, Erwin Adams, Ann Van Schepdael, Jos Hoogmartens

J Chromatogr A. 2004 Nov 12;1056(1-2):111-20.

PMID: 15595540

Abstract:

A simple, isocratic liquid chromatographic (LC) method using volatile mobile phase constituents for the identification of related substances in erythromycin samples is described. For method development, evaporative light scattering detection (ELSD) was used. An XTerra RP18 column was used at 70 degrees C with a mobile phase consisting of acetonitrile-isopropanol-0.2M ammonium acetate pH 7.0-water (165:105:50:680). Mass spectral data were acquired on an ion trap mass spectrometer equipped with an electrospray interface operated in the positive ion mode. First, a library was created using MS/MS and MS(n) spectra of reference substances available in the laboratory. Using these reference spectra as interpretative templates, eight novel related substances in erythromycin samples were identified: N-demethylerythromycin E, erythromycin E N-oxide, anhydroerythromycin C, N-demethylerythromycin B, anhydro-N-demethylerythromycin A, pseudoerythromycin E enol ether, EF lacking the neutral sugar and EA lacking the neutral sugar.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP105882697	Pseudoerythromycin A enol ether		105882-69-7	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: