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Identification of Neuropeptide-Degrading Enzymes in the Pancreas

Identification of Neuropeptide-Degrading Enzymes in the Pancreas

H Terashima, A Okamoto, D Menozzi, E J Goetzl, N W Bunnett

Peptides. Jul-Aug 1992;13(4):741-8.

PMID: 1359509

Abstract:

Neutral endopeptidase (NEP) and aminopeptidase M (APM) were identified in the pancreas by enzymatic assays and Western blotting. The NEP activity, assessed by the phosphoramidon- and DL-thiorphan-inhibitable degradation of glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamine, was 28.8 pmol/h/micrograms of pancreatic membrane protein and 124 pmol/h/10(6) pancreatic acinar cells. The APM enzymatic activity, assessed by the actinonin- and amastatin-inhibitable degradation of Ala-4-methoxy-2-naphthylamine, was 633 pmol/h/micrograms pancreatic membrane protein and 17.4 nmol/h/10(6) pancreatic acinar cells. Proteins corresponding to NEP (95 kDa) and APM (140 kDa) were identified in membranes by Western blotting. Both NEP and APM on acinar cells may degrade neuropeptides and regulate their effects on exocrine secretion.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP76721896-A	DL-Thiorphan		76721-89-6	Price

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