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Identification of Parent Benzodiazepines by Gas chromotography/mass Spectroscopy (GC/MS) From Urinary Extracts Treated With B-glucuronidase

Identification of Parent Benzodiazepines by Gas chromotography/mass Spectroscopy (GC/MS) From Urinary Extracts Treated With B-glucuronidase

S B Needleman, M Porvaznik

Forensic Sci Int. 1995 May 9;73(1):49-60.

PMID: 7750882

Abstract:

Urinary glucuronide metabolites of the benzodiazepines were converted back to the parent molecules after treatment with B-glucuronidase. The benzodiazepines were extracted by a one-step liquid/liquid extraction from urine or by a liquid/solid phase extraction. For the limit of detection (LOD), a standard solution of diazepam and oxazepam was serially diluted and analyzed to the point at which a reproducible analytical result was no longer obtained. Using a temperature program and a splitless mode of injection, excellent quantitation was achieved within an 8-min run time. Based upon specimens obtained from patients under a physician's care, we have determined that urinary concentrations of the benzodiazepines > 200 ng/ml are most likely due to abuse rather than to a prescribed ingestion under strict medical surveillance. Therefore, the calibration standard and cutoff concentration for a positive result was set at 200 ng/ml.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP6801816	Oxazepam glucuronide solution		6801-81-6	Price

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