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Identification of Pin1-binding Phosphorylated Proteins in the Mouse Brain

Identification of Pin1-binding Phosphorylated Proteins in the Mouse Brain

Yota Tatara, Takahiro Terakawa, Takafumi Uchida

Biosci Biotechnol Biochem. 2010;74(12):2480-3.

PMID: 21150095

Abstract:

To determine the role of Pin1 in the neurotransmission pathway, Pin1-binding proteins in mouse brain extract were identified. The Pin1-binding proteins were extracted from mouse brain homogenate, and the trypsin-digested peptides were analyzed by nano-liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins that involve the neurotransmission pathway, such as synapsin I, synapsin II, and calcium/calmodulin-dependent protein kinase type II (CaMKII), were identified in a Mascot search. Pull-down and immunoprecipitation assay indicated that Pin1 binds CaMKII in a phosphorylation-specific manner. It was assumed that Pin1 participates in the neurotransmission pathway involving the phosphorylation signal by CaMKII.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411758	Mouse Brain Extract			Price

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