Home
Resources
Publications
Identification of the Cytolinker Plectin as a Major Early in Vivo Substrate for Caspase 8 During CD95- And Tumor Necrosis Factor Receptor-Mediated Apoptosis

Identification of the Cytolinker Plectin as a Major Early in Vivo Substrate for Caspase 8 During CD95- And Tumor Necrosis Factor Receptor-Mediated Apoptosis

A H Stegh, H Herrmann, S Lampel, D Weisenberger, K Andrä, M Seper, G Wiche, P H Krammer, M E Peter

Mol Cell Biol. 2000 Aug;20(15):5665-79.

PMID: 10891503

Abstract:

Caspase 8 plays an essential role in the execution of death receptor-mediated apoptosis. To determine the localization of endogenous caspase 8, we used a panel of subunit-specific anti-caspase 8 monoclonal antibodies in confocal immunofluorescence microscopy. In the human breast carcinoma cell line MCF7, caspase 8 predominantly colocalized with and bound to mitochondria. After induction of apoptosis through CD95 or tumor necrosis factor receptor I, active caspase 8 translocated to plectin, a major cross-linking protein of the three main cytoplasmic filament systems, whereas the caspase 8 prodomain remained bound to mitochondria. Plectin was quantitatively cleaved by caspase 8 at Asp 2395 in the center of the molecule in all cells tested. Cleavage of plectin clearly preceded that of other caspase substrates such as poly(ADP-ribose) polymerase, gelsolin, cytokeratins, or lamin B. In primary fibroblasts from plectin-deficient mice, apoptosis-induced reorganization of the actin cytoskeleton, as seen in wild-type cells, was severely impaired, suggesting that during apoptosis, plectin is required for the reorganization of the microfilament system.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4241004	Anti-Caspase 9 antibody,Mouse monoclonal			Price
IAR424995	Anti-Caspase 8 antibody, Mouse monoclonal			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: