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Identification of the IGF-1 Processing Product Human Ec/rodent Eb Peptide in Various Tissues: Evidence for Its Differential Regulation After Exercise-Induced Muscle Damage in Humans

Identification of the IGF-1 Processing Product Human Ec/rodent Eb Peptide in Various Tissues: Evidence for Its Differential Regulation After Exercise-Induced Muscle Damage in Humans

George Vassilakos, Anastassios Philippou, Michael Koutsilieris

Growth Horm IGF Res. 2017 Feb;32:22-28.

PMID: 27836414

Abstract:

Objective:
Insulin-like growth factor-1 (IGF-1) is a pleiotropic factor expressed in various tissues and plays a critical role in skeletal muscle physiology. Alternative splicing of the IGF-1 gene gives rise to different precursor polypeptides (isoforms) which could undergo post-translational cleavage, generating the common mature IGF-1 peptide and different carboxyl terminal extension (E-) peptides, with the fate of the latter being, so far, unknown. The objective if this study was to identify the IGF-1Ec forms or processing product(s), other than mature IGF-1, generated in different human and rodent tissues and particularly in human skeletal muscle after exercise-induced damage.
Design:
Protein lysates from a wide range of human and rodent tissues were immunoblotted with a rabbit anti-human Ec polyclonal antibody raised against the last 24 amino acids of the C-terminal of the Ec peptide. This antibody can recognize the Ec peptide, both as part of IGF-1Ec and alone, and also the corresponding rodent forms, due to the high homology that the human Ec shares with the rodent Eb.
Results:
We were able to confirm, for the first time, that the human Ec peptide and its rodent homologous Eb peptide are produced simultaneously with their precursor protein (pro-IGF-1Ec/Eb) in vivo, in a wide range of tissues (e.g. muscle, liver, heart). Proprotein convertase furin digestion of human muscle and liver protein lysates confirmed that the higher molecular form, pro-IGF-1Ec, can be cleaved to produce the free Ec peptide. Furthermore, initial evidence is provided that Ec peptide is differentially regulated during the process of muscle regeneration after exercise-induced damage in humans.
Conclusions:
The findings of this study possibly imply that the post-translational modification of the IGF-1Ec pro-peptide may regulate the bioavailability and activity of the processing product(s).

Chemicals Related in the Paper:

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IAR42414059	Anti-WAVE-1 (C-terminal) antibody produced in rabbit	Price
IAR42417589	Anti-ASK-1, C-terminal antibody produced in rabbit	Price
IAR4246582	Anti-VILIP-1 (C-terminal) antibody produced in rabbit	Price

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