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Idiotype Cascades Associated With the CD4-HIV Glycoprotein 120 Interaction: Immunization With Anti-Idiotypic Antibodies Induces Anti-Anti-Idiotypic Responses With anti-CD4 Specificity and in Vitro Neutralizing Activity

Idiotype Cascades Associated With the CD4-HIV Glycoprotein 120 Interaction: Immunization With Anti-Idiotypic Antibodies Induces Anti-Anti-Idiotypic Responses With anti-CD4 Specificity and in Vitro Neutralizing Activity

M H Shearer, A G Dalgleish, T C Chanh, R C Kennedy

AIDS Res Hum Retroviruses. 2000 Jan 1;16(1):77-86.

PMID: 10628819

Abstract:

Anti-idiotypic antibodies (Ab-2), which were generated in baboons against a mouse monoclonal antibody specific for the CD4 molecule expressed on human T cells, were used to produce anti-anti-idiotypic antibodies (Ab-3) in mice. This response induced by Ab-2 immunization of BALB/c mice was classified as anti-anti-idiotype (Ab-3) on the basis of the ability of the mouse Ab-3 to (1) specifically bind the baboon Ab-2 preparation, but not irrelevant baboon IgG preparations, (2) inhibit the binding of the anti-CD4 Ab-1 preparation to the baboon Ab-2, and (3) recognize a second baboon Ab-2, along with a rabbit Ab-2 specific for the monoclonal anti-CD4 Ab-1 preparation. The murine Ab-3 response also recognizes the CD4 molecule expressed on a human CD4+ T cell line, as determined by flow cytometry; recognizes the same epitopes on the CD4 molecule as the Ab-1; inhibits HIV-1 syncytium formation; and neutralizes HIV-1 primary isolates in vitro. These studies suggest that Ab-3 responses can be induced by anti-Id immunization, which serologically mimicks the antigen and Id specificities of the monoclonal anti-CD4 preparation used to generate the anti-Id. Thus, the Ab-3 response exhibits the characteristics of a population that represents the internal image of the Ab-1.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414709	Anti-CD4 antibody produced in rabbit			Price
IAR4246004	Anti-NKX3-1 (ab2) antibody produced in rabbit			Price

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