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Immobilization of Candida Rugosa Lipase on Glass Beads for Enantioselective Hydrolysis of Racemic Naproxen Methyl Ester

Immobilization of Candida Rugosa Lipase on Glass Beads for Enantioselective Hydrolysis of Racemic Naproxen Methyl Ester

Elif Yilmaz, Keziban Can, Mehmet Sezgin, Mustafa Yilmaz

Bioresour Technol. 2011 Jan;102(2):499-506.

PMID: 20846857

Abstract:

Candida rugosa lipase (CRL) was immobilized on glutaraldehyde-activated aminopropyl glass beads by using covalent binding method or sol-gel encapsulation procedure and improved considerably by fluoride-catalyzed hydrolysis of mixtures of RSi(OCH3)3 and Si(OCH3)4. The catalytic properties of the immobilized lipases were evaluated into model reactions, i.e. the hydrolysis of p-nitrophenylpalmitate (p-NPP). It has been observed that the percent activity yield of the encapsulated lipase was 166.9, which is 5.5 times higher than that of the covalently immobilized lipase. The enantioselective hydrolysis of racemic Naproxen methyl ester by immobilized lipase was studied in aqueous buffer solution/isooctane reaction system and it was noticed that particularly, the glass beads based encapsulated lipases had higher conversion and enantioselectivity compared to covalently immobilized lipase. In short, the study confirms an excellent enantioselectivity (E>400) for the encapsulated lipase with an ee value of 98% for S-Naproxen.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP26159353	(S)-Naproxen methyl ester		26159-35-3	Price

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