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Immobilization of Carbonic Anhydrase on Polyvinylidene Fluoride Membranes

Immobilization of Carbonic Anhydrase on Polyvinylidene Fluoride Membranes

Jing Sun, Lina Wei, Yanzi Wang, Zhiping Zhao, Wenfang Liu

Biotechnol Appl Biochem. 2018 May;65(3):362-371.

PMID: 29222863

Abstract:

In recent years, the application of carbonic anhydrase (CA) in CO2 removal has attracted great interest. However, obtaining high enzyme recovery activity is difficult in existing immobilization techniques. In this work, water plasma-treated poly(vinylidene fluoride) (PVDF) membranes were modified via 3-aminopropyl triethoxy silane (KH550) or γ-(2, 3-epoxypropoxy) propyl trimethoxy silane (KH560), and then CA was attached. The immobilization process was optimized, and the catalytic properties of PVDF-attached CA were characterized. The maximum activity recovery of PVDF-KH550-CA was 60%, whereas that of PVDF-KH560-CA was 33%. The Km values of PVDF-KH550-CA, PVDF-KH560-CA, and free enzyme were 9.97 ± 0.37, 12.5 ± 0.2, and 6.18 ± 0.23 mM, respectively, and their Kcat /Km values were 206 ± 2, 117 ± 5, and 488 ± 4 M-1 ·Sec-1 . PVDF-attached CA shows excellent storage stability and reusability, and their half-life values were 82 and 78 days at 4 °C. At 25 °C, they were 50 and 37 days, respectively. PVDF-KH550-CA and PVDF-KH560-CA retained approximately 85% and 72% of the initial activity after undergoing 10 cycles. In the presence of them, the generation rates of CaCO3 were 76% and 65% of the free CA system, which were 1.6 and 1.3 times that of the blank system, respectively. Its role in accelerating CO2 sequestration holds great promise for its practical application.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP90260870	Triethoxy(1-phenylethenyl)silane		90260-87-0	Price

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