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Immobilization of Rennet From Mucor Miehei via Its Sugar Chain. Its Use in Milk Coagulation

Immobilization of Rennet From Mucor Miehei via Its Sugar Chain. Its Use in Milk Coagulation

Benevides C C Pessela, Rodrigo Torres, Manuel Fuentes, Cesar Mateo, Roberto Fernandez-Lafuente, Jose M Guisán

Biomacromolecules. Sep-Oct 2004;5(5):2029-33.

PMID: 15360320

Abstract:

A successful strategy for the immobilization of rennet from Mucor miehei has been developed. The strategy is based on the immobilization of the enzyme, via their sugar chains at high ionic strength on aminated supports having primary amino groups with a very low pK value. The rennet was covalently immobilized via sugar chains (previously oxidized with periodate), which act as natural spacer arms and allow a very high percentage of rennet activity to be kept against small (H-Leu-Ser-p-nitro-Phe-Nle-Ala-Leu-OMe.TFA (98%)) and macromolecular substrates (k-casein) (78%). The use of tailor-made aminated support was critical to obtain good stability values, because using fully aminated supports achieved much lower thermostability values than using 50% aminated supports. The optimized derivative was utilized to hydrolyze casein in milk. To prevent the coagulation of the milk in the presence of the derivative, the reaction was performed at 4 degrees C (where hydrolyzed casein did not precipitate). Then the hydrolyzed milk was filtered and latter on heated to 30 degrees C, achieving a similar aggregate to the one achieved with soluble rennet.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9042084	Rennet from Mucor miehei		9042-08-4	Price

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