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Immunochemical Studies on Thymosin: Radioimmunoassay of Thymosin Alpha 1

Immunochemical Studies on Thymosin: Radioimmunoassay of Thymosin Alpha 1

J E McClure, N Lameris, D W Wara, A L Goldstein

J Immunol. 1982 Jan;128(1):368-75.

PMID: 7054279

Abstract:

A sensitive and specific radioimmunoassay (RIA) has been developed to measure thymosin alpha 1 in human blood and tissue extracts. Thymosin alpha 1, originally isolated from bovine thymosin fraction 5, has an identical primary structure in bovine and human species. Antiserum to synthetic thymosin alpha 1 was prepared in rabbits. A synthetic analogue, N-Ac-(Tyr1)thymosin alpha 1 was radioiodinated by utilizing Na125I and soluble lactoperoxidase. The RIA is capable of detecting as small an amount as 40 pg thymosin alpha 1 in serum/plasma and does not significantly cross-react with other putative thymic hormones or other purified blood proteins that have been assayed. Proteolytic degradation of thymosin alpha 1 does not occur during the collection, storage, or assay of serum or plasma. The concentration of thymosin alpha 1 in the blood is highest in utero, decreases sharply after birth, and appears to remain fairly constant during the first 15 yr of life. Maternal blood levels of thymosin alpha 1 appear to be above normal during pregnancy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP62304987	Thymosin α1 bovine		62304-98-7	Price

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