Home
Resources
Publications
Immunocomplex-immobilization Technique

Immunocomplex-immobilization Technique

S Oscarsson, J Carlsson

Analyst. 1991 Aug;116(8):787-91.

PMID: 1789459

Abstract:

An immunoassay technique is presented, which works equally well with either a radioactive isotope or an enzyme as the label. It is based on the competition between a labelled antigen of known concentration and an unlabelled antigen (concentration to be determined) for binding to an antibody, which previously has been thiolated by the heterobifunctional reagent N-[3-(2-pyridyldithio)propionyl]succinamide [marketed as N-succinimidyl-3-(2-pyridyldithio)propionate; SPDP)]. The soluble immunocomplex formed is then immobilized onto agarose beads containing reactive disulphide groups. After washing, to remove any unbound and non-specifically adsorbed material, the radioactivity or the enzymic activity on the agarose beads is determined and the concentration of the antigen in the test sample calculated according to the conventional procedure for a competitive immunoassay. In order to demonstrate the applicability of the technique a test procedure for the determination of total serum immunoglobulin E is presented.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS7411171	Succinimidyl Succinamide-PEG5K-Succinimidyl Succinamide			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: